Apoptosis - Triggering Effects: UVB-irradiation and Saccharomyces cerevisiae.
نویسندگان
چکیده
UNLABELLED OBJECTIVES The pathogenic disturbance of Saccharomyces cerevisiae is known as a rare but invasive nosocomial fungal infection. This survey is focused on the evaluation of apoptosis-triggering effects of UVB-irradiation in Saccharomyces cerevisiae. MATERIALS AND METHODS The well-growth colonies of Saccharomyces cerevisiae on Sabouraud Dextrose Agar (SDA) were irradiated within an interval of 10 minutes by UVB-light (302 nm). Subsequently, the harvested DNA molecules of control and UV-exposed yeast colonies were run through the 1% agarose gel electrophoresis comprising the luminescent dye of ethidium bromide. OUTCOMES No unusual patterns including DNA laddering bands or smears were detected. CONCLUSIONS The applied procedure for UV exposure was not effective for inducing apoptosis in Saccharomyces cerevisiae. So, it needs another UV-radiation protocol for inducing apoptosis phenomenon in Saccharomyces cerevisiae.
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عنوان ژورنال:
- Maedica
دوره 7 4 شماره
صفحات -
تاریخ انتشار 2012